Webalkynyl-silylated SM20, which was further dispersed in 100 μL mixture of 60 μM BTTAA, 25 μM CuSO4, 0.5 μM azide-Cy3-Cx and 250 μM sodium ascorbate to incubate with shaking in the dark at room temperature for 2 hours. The cell mimic was prepared by incubating the epoxy-silylated SM10 in 0.8 Web12 Jan 2024 · In Vitro: BTTAA is a Cu(I)-stabilizing ligand. Using the Glu18AzF mutant of ubiquitin as a model system with C3-Tm3+ and C4-Tm3+, Cu-BTTAA performs …
BTTAA CAS 1334179-85-9 AbMole BioScience BTTAA Price
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Comparative Analysis of Click Chemistry Mediated Activity-Based …
WebTBTA. Triborough Bridge and Tunnel Authority. Governmental » Transportation. Rate it: Webwith 100 μM alkyne-biotin, premixed BTTAA-CuSO4 complex (100 μM CuSO4, BTTAA: CuSO4 at a molar ratio of 2:1), and 2.5 mM sodium ascorbate for 1 h. By adding 20 mL ice-cold methanol, the proteins were precipitated in - 30 °C overnight. The proteins were centrifuged at 4,500 × g for 15 min, and washed twice with 20 mL ice-cold methanol. WebBTTP is the latest generation of accelerating ligands for CuAAC, which provides greater rate enhancement compared to previous generation ligands such as THPTA or TBTA. More importantly, it minimizes disturbance to the physiological state of the probed cells or organisms, and enables efficient bioconjugation and inhibition of cytotoxicity by further … tibor translation