WebJul 26, 2024 · columns: I have tested this method on both an Agilent Poroshell 120 HILIC-Z (10 cm) and a Thermo Fisher Accucore HILIC (10 cm). Both columns show the same carryover issues. ... You may want to search for Agilent Poster WP526 from the ASMS 2024, for more inputs (even if the paper is for anions. but you may adapt the idea to your … WebMar 19, 2024 · Hydrophilic interaction chromatography is a type of mixed-mode liquid chromatography, mainly used for the separation of polar compounds. When HILIC first appeared, it was considered a new type of normal phase chromatography, however, now it is thought to be much more complex than being just another type of normal phase …
Problematic polar analytes? Hello HILIC… - Wiki
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InfinityLab Poroshell 120 HILIC Columns Agilent
WebWe also started seeing them after running amino acid standards using a HILIC-Z column. We have a 1260 Infinity II HPLC but coupled to a 6460 triple quad MS. We use a gradient with 90:10 ACN:H20 and H20 both with 10 mM ammonium formate and 0.1% formic acid. WebApr 13, 2024 · HPLC was carried out on an Agilent Poroshell 120 HILIC-Z (100 × 2.1 mm ID, 2.7 µm) column connected to an Agilent Infinity 1290 HPLC. The solvent system was A: 25 mM of ammonium formate and 0.1% formic acid in H 2 O and B: Acetonitrile. The gradient program began and stayed with 97% B during 1 min, then ramped to 60% B at 13 min, … WebApr 13, 2024 · The column of Waters XBridge BEH Amide (c, 2.5 μm, 100 × 3.0 mm) was chosen ultimately, and the others used in the pre-experiments were Agilent Poroshell 120 HILIC-Z (a, 2.7 µm, 100 × 2.1 mm), Waters CORTECS UPLC HILIC (b, 1.6 µm, 150 × 2.1 mm), Waters ACQUITY UPLC BEH Amide (d, 1.7 μm, 100 × 3.0 mm), Phenomenex Kinetex HILIC … simple tops for full breasted women