Phosphoproteome of resting human platelets
WebFor proteomics (global, phospho, N termini) analysis, platelet samples (5.0 × 10 8 /ml) were collected into 1 volume of lysis buffer (50 m m Tris, 1% SDS, 150 m m NaCl, 1 tablet PhosStop/7 ml, pH 7.8) ( 15. ). Lysed samples were immediately frozen and … WebSharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 ... Zahedi RP, et al. (2008) Phosphoproteome of resting human platelets. J Proteome Res 7, 526-34
Phosphoproteome of resting human platelets
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WebCASS4 signaling may contribute to platelet activation and aggregation. A PKA / PKG phosphorylation site has been identified in CASS4 on residue S305 in the unstructured domain containing SH2 -binding motifs; the functional significance of this phosphorylation is currently unknown. [24] WebDec 1, 2006 · Phosphoproteomics of human platelets: A quest for novel activation pathways - ScienceDirect Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics Volume 1764, Issue 12, December 2006, Pages 1963-1976 Review Phosphoproteomics of human platelets: A quest for novel activation pathways René P. Zahedi a , Antonija J. Begonja b , …
WebNov 12, 2024 · (A) Workflow summary for multiplexed, quantitative phosphoproteomics analysis of resting vs CRP-XL-activated platelets. Blood was drawn from n = 5 healthy human donors to prepare washed platelets (>99.9% CD41 + by flow cytometry). Platelets were incubated with apyrase, indomethacin, and Integrilin to inhibit feedback processes …
WebJan 1, 2007 · Phosphoproteomic is an omics approach that helps to characterize the networks of functional proteins during the physiological and pathophysiological processes and their action [123].... WebHuman (Homo sapiens). General Annotation. Sub Unit: The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3.
WebOct 1, 2016 · These lists contain considerable overlap with the phosphoproteome changes reported for human platelets activated with thrombin or oxidized phospholipids (16). …
WebApr 13, 2024 · After 27 days of bed rest, platelet HSP47 was drastically reduced whereas GPVI or GPIbα surface expression did not change (Fig. 5M and fig. S6, G to H). In a metaproteome analysis, we performed a bioinformatic matching for homologous proteins between the human and bear proteome (Fig. 5N and table S5). This enabled a cross … fnf online no download mobile gameWeb1 day ago · Credit: Tobias Petzold, Ludwig-Maximilians University Munich. Bears in hibernation as well as paraplegic people spend months or even years lying almost motionless while avoiding dangerous blood ... greenview medical groupWebDec 20, 2007 · Sigma-Aldrich offers abstracts and full-text articles by [René P Zahedi, Urs Lewandrowski, Julia Wiesner, Stefanie Wortelkamp, Jan Moebius, Claudia Schütz, Ulrich Walter, Stepan Gambaryan, Albert Sickmann]. greenview medical centre hsr layoutWebJul 9, 2024 · Analysis of the platelet phosphoproteome revealed 2,516 phosphorylation sites, of which 453 were regulated by Gsα-PKA. Common changes in the patients were: (1) a joint panel of upregulated and downregulated phosphopeptides; (2) overall PKA dependency of the upregulated phosphopeptides; (3) links to key platelet function pathways. greenview medical center bangaloreWebNov 5, 2024 · We analyzed the phosphoproteome of equivalent amounts of protein extracted from isolated resting platelets from 12 obese patients (4 pools of 3 individuals each) and … greenview medical center kyWebDec 20, 2007 · Sigma-Aldrich offers abstracts and full-text articles by [René P Zahedi, Urs Lewandrowski, Julia Wiesner, Stefanie Wortelkamp, Jan Moebius, Claudia Schütz, Ulrich … fnf online onlineWebTo gain a deeper insight into platelet function, we analyzed the phosphoproteome of resting platelets and identified 564 phosphorylation sites from more than 270 proteins, of which … fnf online pibby mod