site stats

The his tag antibody fitc mab mouse

WebThe antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells. Expand Description Specifications Description: Anti-6X HIS EPITOPE TAG (MOUSE) Monoclonal Antibody - … WebThis antibody was raised against a linear 8XHis tag. It reacts with His-tagged protein with C, N or internal 6XHis tags or longer. It can be used for western blotting to detect linear His repeats (6XHis or more). It can also be used for ICFC and ICC staining of His-tagged proteins. Product Details Technical data sheet Product Details

FITC anti-His-Tag, His Tag, J095G46 - BioLegend

WebAfter washing with BD Perm/Wash™ Buffer (Cat. No. 554723), the cells were stained with either FITC Mouse Anti-Human CD68 antibody (Cat. No. 562117; solid line histogram) or with a FITC Mouse IgG2b, κ Isotype Control (Cat. No. 555057; dashed line histogram). The fluorescence histograms were derived from events with the forward and side light ... hermes ss22 https://deleonco.com

THE™ His Tag Antibody, mAb, Mouse - GenScript

WebMonoclonal antibody (clone #10B8) of IgG isotype specific to hantavirus Gc glycoprotein was generated. ... (His) tag and the Gc-protein was excised using Spe I and subcloned into the Xba I site of the yeast expression vector pFX7 ... Detection was done by incubation with a fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse ... WebOct 6, 2024 · His-Tag Antibody (H-3) is a mouse monoclonal IgG 1 κ His-Tag antibody, cited in 497 publications, provided at 200 µg/ml. raised against a His-tagged recombinant … WebSf21 monolayers were permeabilized for 10 minutes at room temperature (RT) with 0.5% TritonX-100, and then incubated with monoclonal antibody anti-p30 FITC diluted 1:200 (provided by Pestivirus and Asfivirus National Reference Centre) in PBS for 45 minutes at RT. Plates were monitored using a fluorescence microscope. hermes staff

His Tag Monoclonal Antibody (6G2A9) (A00186-100)

Category:Anti-His tag Antibody (HRP), Mouse Monoclonal - Sinobiological

Tags:The his tag antibody fitc mab mouse

The his tag antibody fitc mab mouse

6x-His Tag Monoclonal Antibody (AD1.1.10), FITC

WebThe His-tag antibody was raised using a 6-His tag peptide as the immunogen, which is ~1 kDa. Some scientists use more than 6 histidine residues as a tag for recombinant proteins to increase affinity to metal ions. A 6-histidine tag is sufficient for specific recognition by the His-tag antibody. WebApr 11, 2024 · PBMCs were stained with S-trimer-Strep-tag, CD19-APC-Cy7 (BioLegend, #302217), and IgD-FITC (BioLegend, #348206) for 20 min on ice. ... Single-cell RT-PCR and monoclonal antibody production ...

The his tag antibody fitc mab mouse

Did you know?

WebHis-Tag (27E8) Mouse mAb detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of … WebDescription; Overview: The His•Tag ® Monoclonal Antibody is a mouse monoclonal antibody (IgG 1) directed against the His•Tag sequence encoded by many of the Novagen ® expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid …

WebA His-tag (polyhistidine tag) consists of at least six histidine residues that are located at the N- or C-terminus of recombinant proteins. It is commonly used for affinity purification and … WebHis Tag Antibodies. His-tag is a series of six to nine histidine residues fused to either the carboxyl or amino terminus of a recombinant protein. The small size of the His-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions.

WebTHE TM His Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins. Characteristics Anti-His mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. WebGenScript THE™ His Tag Antibody [FITC], mAb, Mouserecognizes C-terminal, N-terminal, and internal His tagged fusion proteins. Product Details Citations Documents Overview …

WebHis-Tag (27E8) Mouse mAb detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. The antibody may cross-react with a protein of unknown origin ~60-70kDa. Species Reactivity: All Species Expected Source / Purification

Web6x-His Tag Monoclonal Antibody (HIS.H8), DyLight™ 650, Invitrogen™-Mouse Monoclonal Antibody Compre 6x-His Tag Monoclonal Antibody (HIS.H8), DyLight™ 650, max bather loadWebGenScript THETM His Tag Antibody [FITC], mAb, Mouse is an FITC conjugated THETM His Tag Antibody, mAb, Mouse (A00186) purified from mice ascites by Protein A affinity … maxbass nd to minot ndWebOct 6, 2024 · His-Tag Antibody (H-3) is an IgG 1 κ mouse monoclonal His-Tag antibody (also designated 6X His-Tag antibody or His-Probe antibody) suitable for the detection of the His-Tag protein. His-Tag Antibody (H-3) is available as both the non-conjugated anti-His-Tag antibody form, as well as multiple conjugated forms of anti-His-Tag antibody, … hermes staff imagesWebCD70 Background Information. CD70, a member of the tumor necrosis factor superfamily, is restricted to activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor, CD27, is important in priming, effector functions, differentiation and memory formation of T-cells as well as plasma and memory B-cell generation. max batchesWebTHE™ His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags. This … maxbass nd historic photosWebThis antibody was raised against a linear 8XHis tag. It reacts with His-tagged protein with C, N or internal 6XHis tags or longer. It can be used for western blotting to detect linear His … maxbass north dakotaWebHIS Tag Antibody Mouse Monoclonal Antibody (Mab) Anti-HIS Tag at 1:8000 dilution + 12-tag lysate Lysates/proteins at 20ng per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST. SPECIFICATION CITATIONS: 50 PROTOCOLS BACKGROUND … max batch size in salesforce